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| Registros recuperados : 14 | |
| 1. |  | LEASTRO, M. O.; PAZ LIMA, M. L.; SILVA, C. F. Aspectos morfofisiologicos de isolados de Myrothecium roridum. Tropical Plant Pathology, Brasília, DF, v. 34, p. S180, 2009. Suplemento. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | LEASTRO, M. O.; KITAJIMA, E. W.; SILVA, M. S.; RESENDE, R. O.; ASTUA, J. de F. Dissecting the subcellular localization, intracellular trafficking, interactions, membrane association, and topology of Citrus Leprosis Virus. C proteins. Frontiers in Plant Science, v. 19, 2018.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | MACEDO, M. A.; PEREIRA, T. G.; LEASTRO, M. O.; INOUE-NAGATA, A. K. Efeito de adubação e do hospedeiro na expressão de sintomas causados por begomovírus em plantas de tomate. Tropical Plant Pathology, Brasília, DF, v. 35, p. S281, ago. 2010. Suplemento. Resumo 11.050. Trabalho apresentado no 43. Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | LEASTRO, M. O.; OLIVEIRA, A. S.; LIMA, M. F.; RESENDE, R. O. Caracterização da proteína supressora de silenciamento gênico (NSs) do Zucchini lethal chlorosis. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 835.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | HALLWASS, M.; LEASTRO, M. O.; LIMA, M. F.; INOUE-NAGATA, A. K.; RESENDE, R. O. Sequence determination and analysis of the NSs genes of two tospoviruses. Archives of Virology, New York, v. 157, n. 3, p. 591-596, Mar. 2012.| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Citrus LEPROSIS virus c codifica três proteínas com atividades supressoras de silenciamento gênico Citrus Leprosis Virus C encodes three proteins with gene silencing suppression activity. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 190. Evento online.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 14 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2021 |
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Data da última atualização: |
09/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia. |
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Título: |
Unravelling the involvement of cilevirus p32 protein in the viral transport. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientifc Reports, n.11, v.2943, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. MenosCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus NAL: |
Citrus; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2129841
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100 1 $aLEASTRO, M. O.
245 $aUnravelling the involvement of cilevirus p32 protein in the viral transport.$h[electronic resource]
260 $c2021
520 $aCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.
650 $aCitrus
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aNAVARRO, J. S.
773 $tScientifc Reports$gn.11
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